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つくばリポジトリ (Tulips-R) >
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Plant physiology and biochemistry >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/2241/116860
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| Title: | Molecular and physiological dissection of enhanced seed germination using short-term low-concentration salt seed priming in tomato |
| Authors: | Nakaune, Makoto
Hanada, Atsushi
Yin, Yong-Gen
Matsukura, Chiaki
Yamaguchi, Shinjiro
Ezura, Hiroshi
松倉, 千昭
江面, 浩 |
| Issue Date: | Mar-2012 |
| Publisher: | Elsevier Masson SAS. |
| Journal Title: | Plant physiology and biochemistry |
| Volume: | 52 |
| Start Page: | 28 |
| End Page: | 37 |
| DOI: | 10.1016/j.plaphy.2011.11.005 |
| PMID: | 22305065 |
| Abstract: | Seed germination is the initial step of plant development. Seed priming with salt promotes seed germination in tomato (Solanum lycopersicum L.); however, the molecular and physiological mechanisms underlying the enhancement of seed germination by priming remain to be elucidated. In this study, we examined the following in seeds both during and after priming treatment: the endogenous abscisic acid (ABA) and gibberellin (GA) concentrations; the expression of genes encoding ABA catabolic and GA biosynthesis enzymes, including 8′-hydroxylase (CYP707A), copalyl diphosphate synthase (CPS), GA 20-oxidase (GA20ox) and GA 3-oxidase (GA3ox); and endosperm cap weakening enzymes, including expansin (EXP), class I β-1,3-glucanase (GulB), endo-β-mannanase (MAN) and xyloglucan endotransglucosylase (XTH). Tomato seeds were soaked for 24 h at 25 °C in the dark in 300 mM NaCl (NaCl-priming) or distilled water (hydro-priming). For both priming treatments, the ABA content in the seeds increased during treatment but rapidly decreased after sowing. Both during and after the priming treatments, the ABA levels in the hydro-primed seeds and NaCl-primed seeds were not significantly different. The expression levels of SlGA20ox1, SlGA3ox1 and SlGA3ox2 were significantly enhanced in the NaCl-primed seeds compared to the hydro-primed seeds. The GA4 content was quantifiable after both types of priming, indicating that GA4 is the major bioactive GA molecule involved in tomato seed germination. The GA4 content was significantly higher in the NaCl-primed seeds than in the hydro-primed seeds 12 h after sowing and thereafter. Additionally, the peak expression levels of SlEXP4, SlGulB, SlMAN2 and SlXTH4 occurred earlier and were significantly higher in the NaCl-primed seeds than in the hydro-primed seeds. These results suggest that the observed effect of NaCl-priming on tomato seed germination is caused by an increase of the GA4 content via GA biosynthetic gene activation and a subsequent increase in the expression of genes related to endosperm cap weakening. |
| URI: | http://hdl.handle.net/2241/116860 |
| Rights: | © 2011 Elsevier Masson SAS. NOTICE: this is the author’s version of a work that was accepted for publication in Plant physiology and biochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, VOL52, March 2012, DOI:10.1016/j.plaphy.2011.11.005 |
| Text Version: | author |
| Appears in Collections: | 松倉 千昭 (Matsukura Chiaki)
江面 浩 (Ezura Hiroshi)
Plant physiology and biochemistry
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