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つくばリポジトリ (Tulips-R) >
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Zoological science >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/2241/104115
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| Title: | Association of a 66 kDa Homolog of Chlamydomonas DC2, a Subunit of the Outer Arm Docking Complex, with Outer Arm Dynein of Sperm Flagella in the Ascidian Ciona intestinalis |
| Authors: | Ushimaru, Yuji
Konno, Aru
Kaizu, Maiko
Ogawa, Kazuo
Satoh, Nori
Inaba, Kazuo
稲葉, 一男 |
| Issue Date: | Sep-2006 |
| Publisher: | 日本動物学会 |
| Journal Title: | Zoological science |
| Volume: | 23 |
| Issue: | 8 |
| Start Page: | 679 |
| End Page: | 687 |
| DOI: | 10.2108/zsj.23.679 |
| PMID: | 16971786 |
| Abstract: | We previously identified a 66 kDa axonemal protein (Ci-Axp66.0) in sperm of the ascidian Ciona intestinalis. Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chlamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66.0 suggested that the N-terminal two-thirds of the molecule is rich in coiled coil structure, as in Chlamydomonas DC2. Immunogold localization revealed that it is located in the vicinity of outer arm dynein. Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. This co-purification was not affected by the absence of Mg2+ in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. These results suggest that Ci-Axp66.0 is a component of the outer arm dynein docking complex in the axonemes of Ciona sperm. |
| URI: | http://hdl.handle.net/2241/104115 |
| Rights: | © 2006 Zoological Society of Japan |
| Text Version: | publisher |
| Appears in Collections: | Zoological science
稲葉 一男 (Inaba Kazuo)
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